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rabbit anti-rat collagen type iii  (Millipore)


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    Structured Review

    Millipore rabbit anti-rat collagen type iii
    Rabbit Anti Rat Collagen Type Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-rat+collagen+type+iii/rabbit+anti+collagen+iv/pmc02874011-104-13-18
    Average 90 stars, based on 1 article reviews
    rabbit anti-rat collagen type iii - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.

    RNA Extraction:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.

    SYBR Green Assay:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.

    Diagnostic Assay:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.

    Amplification:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.

    Gel Extraction:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.

    Synthesized:

    Article Title: Ultrastructural, immunohistochemical and biochemical investigations of the rat liver exposed to experimental diabetes und acute hypoxia with and without application of Ginkgo extract.
    Article Snippet: The aim of this paper was to investigate the effect of streptozotocin-induced diabetes by i.p. bolus injection of streptozotocin at 60 mg per kg bodyweight over four months and additional acute respiratory hypoxia (20 min. duration, 5% oxygen v/v), and also the protective effect of Ginkgo biloba extract (EGb 761) on Wistar rat liver under these experimental conditions.. Diabetic and additional hypoxic alterations in histology and ultrastructure were subjected to qualitative and quantitative analysis, collagen was investigated by immunohistochemistry, and some biochemical parameters of oxidative stress were determined.. Diabetes caused an increase in the size of the hepatocytes and their nuclei with a decrease in nucleus-to-plasma ratio and glycogen content.

    Article Title: Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells
    Article Snippet: Schneider, Eric, Alexandra Schmid-Kotsas, Jinshun Zhao, Hans Weidenbach, Roland M. Schmid, Andre Menke, Guido Adler, Johannes Waltenberger, Adolf Grünert, and Max G. Bachem.. Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells.. Am J Physiol Cell Physiol 281: C532–C543, 2001.—The aim of this study was to identify fibrogenic mediators stimulating activation, proliferation, and/or matrix synthesis of rat pancreatic stellate cells (PSC).

    Article Title: Major basic protein-1 promotes fibrosis of dystrophic muscle and attenuates the cellular immune response in muscular dystrophy
    Article Snippet: Sections were incubated with primary antibodies for 3 h and with host-appropriate fluorescent-conjugated secondary antibodies (Vector) for 1 h. Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), and goat anti-human collagen type IV (Southern Biotech, Birmingham, AL, USA).

    Article Title: Arginine Metabolism by Macrophages Promotes Cardiac and Muscle Fibrosis in mdx Muscular Dystrophy
    Article Snippet: Primary antibodies used were rabbit anti-rat collagen type I (Chemicon, Temecula, CA, USA), rabbit anti-rat collagen type III (Chemicon), goat anti-human collagen type IV (Southern Biotech), and goat anti-human collagen type V (Southern Biotech).

    Article Title: Ginkgo biloba extract protects rat kidney from diabetic and hypoxic damage.
    Article Snippet: Ginkgo biloba extract EGb 761 was studied for its nephroprotective effects in experimentally diabetic and hypoxic rats.. Duration of streptozotocin-induced diabetes was 4 months, that of respiratoric hypoxia of the diabetic group 20min.. The daily dose of 100mg EGb/kg bodyweight started 1 month after induction of the diabetes.



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    Image Search Results


    Figure 4 RCDs/UA@Lipo alleviated H2O2-induced oxidative stress and TGF-β1 induced fibrosis-specific markers separately. (A) Immunofluorescence staining images of intracellular ROS and superoxide detected by DCFH-DA probe. (B) mitochondrial membrane potential (ΔΨm) detected by JC-1 probe. (C) tendon fibrosis markers (COL I, COL III, α-SMA) of NIH3T3. (D–H) Semiquantitative analysis of the relative fluorescent intensity of (A–C) (n = 3). (D and E) Model group is H2O2 group, (F–H) Model group is TGF-β1 group. Data are presented as mean ± SD; comparisons between the groups were performed one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.

    Journal: International Journal of Nanomedicine

    Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

    doi: 10.2147/ijn.s466312

    Figure Lengend Snippet: Figure 4 RCDs/UA@Lipo alleviated H2O2-induced oxidative stress and TGF-β1 induced fibrosis-specific markers separately. (A) Immunofluorescence staining images of intracellular ROS and superoxide detected by DCFH-DA probe. (B) mitochondrial membrane potential (ΔΨm) detected by JC-1 probe. (C) tendon fibrosis markers (COL I, COL III, α-SMA) of NIH3T3. (D–H) Semiquantitative analysis of the relative fluorescent intensity of (A–C) (n = 3). (D and E) Model group is H2O2 group, (F–H) Model group is TGF-β1 group. Data are presented as mean ± SD; comparisons between the groups were performed one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.

    Article Snippet: Evaluation of Anti-Fibrotic Effects of the Biomaterials by Immunofluorescent Staining at Six Weeks The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

    Techniques: Immunofluorescence, Staining, Membrane

    Figure 6 RCDs/UA@Lipo-HAMA reduced adhesion of injured tendons at macroscopic and histological levels at various time points post-injury. (A) Schematic diagram of surgical procedures of the ATI. (B) Gross view of ATI. (C)Representative images of immunofluorescence staining of tendon antioxidant (Nrf-2, HO-1) and anti-inflammatory markers (CD68, iNOS) at 2 weeks post-injury (n = 3). (D) Representative images of immunofluorescence staining of tendon antifibrosis (COL III, α-SMA) at 6 weeks post- injury (n = 3). (E) Representative images of immunohistochemical staining of tendon markers (Vimentin, MMP2, α-SMA) antifibrosis at 6 weeks post-injury (n = 4). (F–N) Semiquantitative analysis of expression level of tendon marker of (C–E), respectively. Data are presented as mean ± SD; comparisons between the groups were performed by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

    doi: 10.2147/ijn.s466312

    Figure Lengend Snippet: Figure 6 RCDs/UA@Lipo-HAMA reduced adhesion of injured tendons at macroscopic and histological levels at various time points post-injury. (A) Schematic diagram of surgical procedures of the ATI. (B) Gross view of ATI. (C)Representative images of immunofluorescence staining of tendon antioxidant (Nrf-2, HO-1) and anti-inflammatory markers (CD68, iNOS) at 2 weeks post-injury (n = 3). (D) Representative images of immunofluorescence staining of tendon antifibrosis (COL III, α-SMA) at 6 weeks post- injury (n = 3). (E) Representative images of immunohistochemical staining of tendon markers (Vimentin, MMP2, α-SMA) antifibrosis at 6 weeks post-injury (n = 4). (F–N) Semiquantitative analysis of expression level of tendon marker of (C–E), respectively. Data are presented as mean ± SD; comparisons between the groups were performed by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: Evaluation of Anti-Fibrotic Effects of the Biomaterials by Immunofluorescent Staining at Six Weeks The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

    Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Expressing, Marker

    Figure 3. Representative histological sections immunostained for collagen types I (a and b) and III (c and d), from surgically severed Achilles tendons in sheep treated with PRGF (a and c) or saline solution (b and d).

    Journal: Journal of Applied Animal Research

    Article Title: Effect of plasma rich in growth factors on the early phase of healing of surgically severed Achilles tendon in sheep: histological study

    doi: 10.1080/09712119.2017.1337017

    Figure Lengend Snippet: Figure 3. Representative histological sections immunostained for collagen types I (a and b) and III (c and d), from surgically severed Achilles tendons in sheep treated with PRGF (a and c) or saline solution (b and d).

    Article Snippet: IHC analysis was performed for collagen type I (Rabbit antibovine collagen type I; Millipore; California, USA) and type III (Rabbit anti-rat collagen type III; AbD Serotec; Kidlington, UK).

    Techniques: Saline